[0A60:36A4][2018-10-10T08:50:15]i000: WPD set_progressBar_value start: startValue:0 endValue:15 incrementMaxValue:1 incrementMinValue:1 sleepValue:5000[0A60:2CF0][2018-10-10T08:50:15]i000: WPF MainModelView - ExecutePackageBegin KM Call Exception:System.DllNotFoundException: Unable to load DLL 'Nitro_KissMetrics.dll': The specified module could not be found. (Exception from HRESULT: 0x8007007E) at Nitro.Bootstrapper.KissMetrics.KM_RecordEvent_FromBootstrapper(String inVersion, String inInstallSource, String inLanguage, String inErrorCode, String inEventName) at Nitro.Bootstrapper.MainViewModel.Call_KM(String inVersion, String inInstallSource, String inLanguage, String inErrorCode, String inEventName) at Nitro.Bootstrapper.MainViewModel.ExecutePackageBegin(Object sender, ExecutePackageBeginEventArgs e)[3D7C:2EC4][2018-10-10T08:50:15]i301: Applying execute package: NitroInstallationPackageId_x64_en, action: Install, path: C:\ProgramData\Package Cache\1DD58739-D7CC-497A-900C-64275F3DDC56v11.0.3.173\nitro_pro11_ba_x64.msi, arguments: ' ARPSYSTEMCOMPONENT="1" MSIFASTINSTALL="7" APPLICATIONFOLDER="C:\Program Files\Nitro\Pro 11" UPDATESERVICE="0" NPDEFAULTVIEWER="1" NPBROWSERPLUGIN="0" OFFICEADDINS="0" InstallLanguage="en" INSTALLSOURCE="retail" ANALYTICSDISABLED="0"'[0A60:36A4][2018-10-10T08:50:16]i000: WPD MainModelView set_progressBar_value Install_Progress:0[3D7C:2EC4][2018-10-10T08:50:19]e000: Error 0x80070643: Failed to install MSI package.[3D7C:2EC4][2018-10-10T08:50:19]e000: Error 0x80070643: Failed to execute MSI package.[0A60:2CF0][2018-10-10T08:50:19]e000: Error 0x80070643: Failed to configure per-machine MSI package.[0A60:2CF0][2018-10-10T08:50:19]i000: WPF MainModelView - ExecutePackageComplete + InstallCanceled:False[0A60:2CF0][2018-10-10T08:50:19]i319: Applied execute package: NitroInstallationPackageId_x64_en, result: 0x80070643, restart: None[0A60:2CF0][2018-10-10T08:50:19]e000: Error 0x80070643: Failed to execute MSI package.[0A60:2CF0][2018-10-10T08:50:19]i000: WPD MainModelView - set MSIInstallStarted:True[0A60:2CF0][2018-10-10T08:50:19]i000: WPF MainModelView - ExecutePackageBegin + InstallCanceled:False[0A60:2CF0][2018-10-10T08:50:19]i000: WPF MainModelView - get inInstallLanguage:enen-US[0A60:2CF0][2018-10-10T08:50:19]i000: Setting string variable 'AnalyticsDisabled' to value '0'[0A60:2CF0][2018-10-10T08:50:19]i000: WPD MainModelView - Call_KM:0:ProBSInstalling[0A60:2CF0][2018-10-10T08:50:19]i000: Setting string variable 'AnalyticsDisabled' to value '0'[0A60:2CF0][2018-10-10T08:50:19]i000: WPF MainModelView - ExecutePackageBegin KM Call Exception:System.DllNotFoundException: Unable to load DLL 'Nitro_KissMetrics.dll': The specified module could not be found. (Exception from HRESULT: 0x8007007E) at Nitro.Bootstrapper.KissMetrics.KM_RecordEvent_FromBootstrapper(String inVersion, String inInstallSource, String inLanguage, String inErrorCode, String inEventName) at Nitro.Bootstrapper.MainViewModel.Call_KM(String inVersion, String inInstallSource, String inLanguage, String inErrorCode, String inEventName) at Nitro.Bootstrapper.MainViewModel.ExecutePackageBegin(Object sender, ExecutePackageBeginEventArgs e)[3D7C:2EC4][2018-10-10T08:50:19]i318: Skipped rollback of package: NitroInstallationPackageId_x64_en, action: Uninstall, already: Absent[0A60:2CF0][2018-10-10T08:50:19]i000: WPF MainModelView - ApplyProgress:0:0[0A60:2CF0][2018-10-10T08:50:19]i000: WPF MainModelView - ExecutePackageComplete + InstallCanceled:False[0A60:2CF0][2018-10-10T08:50:19]i319: Applied rollback package: NitroInstallationPackageId_x64_en, result: 0x0, restart: None[3D7C:2EC4][2018-10-10T08:50:19]i329: Removed package dependency provider: 1DD58739-D7CC-497A-900C-64275F3DDC56, package: NitroInstallationPackageId_x64_en[3D7C:2EC4][2018-10-10T08:50:19]i351: Removing cached package: NitroInstallationPackageId_x64_en, from path: C:\ProgramData\Package Cache\1DD58739-D7CC-497A-900C-64275F3DDC56v11.0.3.173\[3D7C:2EC4][2018-10-10T08:50:19]i329: Removed package dependency provider: C602317C-6E22-4D33-8AF4-82A48D4FB4FF, package: NitroInstallationPackageId_x86_de[3D7C:2EC4][2018-10-10T08:50:19]i329: Removed package dependency provider: 0F088B7C-E593-438F-AABB-1B393864EE78, package: NitroInstallationPackageId_x64_de[3D7C:2EC4][2018-10-10T08:50:19]i329: Removed package dependency provider: 8FAF8667-5453-4FE7-9364-042348B71CC4, package: NitroInstallationPackageId_x86_da[3D7C:2EC4][2018-10-10T08:50:19]i329: Removed package dependency provider: 7EC8C7FA-9D7D-4900-BCF7-F37F64C5B033, package: NitroInstallationPackageId_x64_da[3D7C:2EC4][2018-10-10T08:50:19]i330: Removed bundle dependency provider: 7bf73063-efea-4392-8504-ea9185b42df0[3D7C:2EC4][2018-10-10T08:50:19]i352: Removing cached bundle: 7bf73063-efea-4392-8504-ea9185b42df0, from path: C:\ProgramData\Package Cache\7bf73063-efea-4392-8504-ea9185b42df0\[0A60:2CF0][2018-10-10T08:50:19]i000: WPD MainModelView - OnApplyComplete-2147023293:None[0A60:2CF0][2018-10-10T08:50:19]i000: WPD MainModelView - set ErrorEnabled:True[0A60:3FD0][2018-10-10T08:50:19]i000: WPD Switch - new page:Nitro.Bootstrapper.Pages.Page_Error[0A60:2CF0][2018-10-10T08:50:19]i000: WPD MainModelView - set InstallEnabled:False[0A60:2CF0][2018-10-10T08:50:19]i000: WPD MainModelView - set UninstallEnabled:False[0A60:2CF0][2018-10-10T08:50:19]i000: WPD MainModelView - set ProgressEnabled:False isResources:True[0A60:2CF0][2018-10-10T08:50:19]i000: WPD MainModelView - set InstallCanceled:False[0A60:2CF0][2018-10-10T08:50:19]i000: WPD MainModelView - get ErrorEnabled:True[0A60:2CF0][2018-10-10T08:50:19]i000: WPF MainModelView - get inInstallLanguage:enen-US[0A60:2CF0][2018-10-10T08:50:19]i000: WPF MainModelView - OnApplyComplete KM Call:11.0.3.173, retail, en-US, ProBSInstallError[0A60:2CF0][2018-10-10T08:50:19]i000: WPF MainModelView - get inInstallLanguage:enen-US[0A60:2CF0][2018-10-10T08:50:19]i000: Setting string variable 'AnalyticsDisabled' to value '0'[0A60:2CF0][2018-10-10T08:50:19]i000: WPD MainModelView - Call_KM:0:ProBSInstallError[0A60:2CF0][2018-10-10T08:50:19]i000: Setting string variable 'AnalyticsDisabled' to value '0'[0A60:2CF0][2018-10-10T08:50:19]i000: WPF MainModelView - OnApplyComplete KM Call Exception:System.DllNotFoundException: Unable to load DLL 'Nitro_KissMetrics.dll': The specified module could not be found. (Exception from HRESULT: 0x8007007E) at Nitro.Bootstrapper.KissMetrics.KM_RecordEvent_FromBootstrapper(String inVersion, String inInstallSource, String inLanguage, String inErrorCode, String inEventName) at Nitro.Bootstrapper.MainViewModel.Call_KM(String inVersion, String inInstallSource, String inLanguage, String inErrorCode, String inEventName) at Nitro.Bootstrapper.MainViewModel.OnApplyComplete(Object sender, ApplyCompleteEventArgs e)[0A60:2CF0][2018-10-10T08:50:19]i399: Apply complete, result: 0x80070643, restart: None, ba requested restart: No[0A60:3FD0][2018-10-10T08:50:19]i000: WPD Page_Progress ST_InstallFinished_IsEnabledChanged:Visible:Hidden[0A60:3FD0][2018-10-10T08:50:19]i000: WPD ProgressBarPageProgress_Unloaded[0A60:3FD0][2018-10-10T08:50:19]i000: WPD ProgressBar workerThread Abort5[0A60:3FD0][2018-10-10T08:50:22]i000: WPD MainModelView - get ProgressEnabled:False[0A60:3FD0][2018-10-10T08:50:22]i000: WPD MainModelView - ExitExecute InvokeShutdown:C:\Users\admusmanj\Desktop\nitro_professional8_x64_11-0-3-173.msi[0A60:2CF0][2018-10-10T08:50:22]i500: Shutting down, exit code: 0x0
Nitro Pro 8 serial 318
Abstract:Chiral β-nitroalcohols are important building blocks in organic chemistry. The synthetic approach that is based on the enzyme-mediated reduction of α-nitroketones has been scarcely considered. In this work, the use of commercial alcohol dehydrogenases (ADHs) for the reduction of aromatic and aliphatic nitroketones is investigated. High conversions and enantioselectivities can be achieved with two specific ADHs, affording either the (S) or (R)-enantiomer of the corresponding nitroalcohols. The reaction conditions are carefully tuned to preserve the stability of the reduced product, and to avoid the hydrolytic degradation of the starting substrate. The further manipulation of the enantioenriched nitroalcohols into Boc-protected amminoalcohols is also described. Keywords: nitroketone; reduction; alcohol-dehydrogenase; enantioselectivity
The immunohistochemical and immunocytochemical analyses were principally carried out as previously described (Mäkelä et al, 1999; Prikk et al, 2001). The polyclonal rabbit anti-human MMP-8 does not detect pure human MMP-1, -2, -3, -9, or -13. For immunostaining, the Vectastain Rabbit ABC Elite Kit (Vector Laboratories, Burlingame, California) was used according to the manufacture's instructions. Bronchial biopsies were embedded in OCT-medium and 5-μm serial sections were cut. The frozen bronchial tissue specimens and cytocentrifuged BALF cell slides were melted, air-dried, and fixed in acetone for 10 minutes at 4 C. The endogenous peroxidase activity was blocked by incubation for 30 minutes with 0.6% H2O2 in methanol. The nonspecific binding sites were blocked by incubation with normal goat serum (1:50) for 3 hours. Thereafter, the sections were incubated with polyclonal anti-MMP-8 (Hanemaaijer et al, 1997; Sorsa et al, 1999; diluted 1:1000) overnight at 4 C and then sections were incubated with biotinylated anti-rabbit immunoglobulin G (1:250) for 1 hour and with avidin-biotin complex (1:125) for 30 minutes. The sections were stained with 3-amino-9-ethylcarbazole (AEC) and counterstained with Mayer's hematoxylin. For negative control, the primary antibody was replaced with a correspondent nonimmune serum. 2ff7e9595c
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